Acid-fast bacilli isolated from foot pads of nude mice infected with leprosy bacilli

The Experimental Disease That Follows the Injection of Human Leprosy Bacilli into Foot-pads of Mice

When leprosy bacilli from human patients are inoculated into the foot-pads of CFW mice, a microscopic granuloma containing acid-fast bacilli develops in a characteristic manner. This has been seen in 22 of 22 instances with leprosy bacilli from nasal washings, in 12 of 16 instances with leprosy bacilli from skin biopsies, and in none of 16 cases where the nasal washings were not observed to con...

Acid-fast bacilli in the bone marrow of leprosy patients.

Comparative morphology of acid-fast bacilli.

Safer staining method for acid fast bacilli.

To develop a method for staining acid fast bacilli which excluded highly toxic phenol from the staining solution. A lipophilic agent, a liquid organic detergent, LOC High Studs, distributed by Amway, was substituted. The acid fast bacilli stained red; nuclei, cytoplasm, and cytoplasmic elements stained blue on a clear background. These results compare very favourably with acid fast bacilli stai...

The Cellular Reactions to Lipoid Fractions from Acid-fast Bacilli

1. A comparative study has been made of the cellular reactions induced by phosphatides from five strains of acid-fast bacilli. Each of these reactions is characterized principally by epithelioid cells and giant cells. 2. The phosphatides are first phagocytized by young connective tissue cells or monocytes. The lipoid is then dispersed into fine particles with the formation of classical epitheli...